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KMID : 1040020200110010011
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International Journal of Pain
2020 Volume.11 No. 1 p.11 ~ p.20
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Tannin Inhibits Histamine and SLIGRL-NH2-Induced Itch via TRPV1 and TRPA1 Signaling Pathway
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Jwa Hyeon-Seok
Kim Seung-Hoon
Jeon Ha-Won
Park Gi-Yeon
Choi Jung-Ju
Park Chul-Kyu
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Abstract
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Background: Tannin is a naturally occurring plant polyphenol. Its main characteristic is that it displays anti-oxidant, anti-inflammatory, anti-viral, and anti-proliferative biological activities. However, the mechanism by which tannin impacts itch remains unknown. In the present study, through behavioral analysis and calcium imaging, we examined the mechanism by which tannin reduces histaminergic itch and non-histaminergic itch.
Methods: First, we confirmed that tannin inhibited itch in the scratching test with histamine, compound 48/80, and SLIGRL-NH2 treatment. Second, we confirmed that tannin inhibited capsaicin- and AITC-induced calcium responses in primary-cultured DRG neurons. Third, tannin blocked the capsaicin- and AITC-induced calcium responses in TRPV1- and TRPA1-expressing HEK293 cells, respectively.
Results: These results reveal that tannin reduced histamine-dependent or independent itching by activating inhibitory signals of TRPV1 and TRPA1.
Conclusions: Thus, our findings suggest that tannin may be a promising drug for the treatment of histamine or non-histaminedependent itch.
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KEYWORD
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tannin, itch, TRPV1, TRPA1
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